Figure 5

Cytokine induction by central nervous system antigen presenting cells in primed CD4 T cells. Fluorescence-activated cell sorted CD4⁺ T cells from myelin oligodendrocyte glycoprotein (MOG)_p35–55 immunised B6 mice with severe experimental autoimmune encephalomyelitis were co-cultured with antigen presenting cells (CD11c⁻ microglia, CD11c⁺ microglia, CD45^high CD11c⁺ cells and spleen-derived dendritic cells (spl. DC) in the presence of MOG_p35–55 peptide. The cytokines IFN-γ and IL-17A from supernatants were quantified by cytokine bead array. Data are presented as means ± SEM of three individual experiments (n ≥ 9). ns, Not significant; *P < 0.05; **P < 0.01.