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Figure 1 | Journal of Neuroinflammation

Figure 1

From: Deoxyglucose prevents neurodegeneration in culture by eliminating microglia

Figure 1

Deoxyglucose prevents spontaneous neuronal loss in vitro. (A) Density of live, necrotic and apoptotic neurons in neuronal–glial cultures at 11 days in vitro (DIV) depicted ±10 mM deoxyglucose added at 7 DIV. (B) Density of live microglia in the same experiment. (C) Density of live astrocyes in the same experiment. (D) Sample fluorescent images of the same experiment stained with Hoescht 33342 to identify nuclei. (E) The same untreated field imaged in phase contrast brightfield at 7 and 11 DIV, showing neuronal loss. Data presented as mean ± standard error of the mean for ≥ 3 independent experiments. Statistically significant differences between live neuronal densities: ***P < 0.001, ****P < 0.0001.

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