Figure 6
From: Deoxyglucose prevents neurodegeneration in culture by eliminating microglia
Deoxyglucose rapidly depletes ATP prior to microglial death. (A) BV-2 density at different time points (0, 1 hour, 3 hours, 6 hours, 24 hours, 48 hours) after addition of 10 mM deoxyglucose (DOG) measured by flow cytometry. (B) Cellular ATP levels determined with luciferin/luciferase at the same time points. RLU, relative light units. (C) Percentage of Annexin V-positive BV-2 cells measured by flow cytometry at the same time points. (D) Percentage of propidium iodide (PI)-positive BV-2 cells measured by flow cytometry at the same time points. Data presented as mean ± standard error of the mean for ≥ 3 independent experiments.*P < 0.05, ***P < 0.001, ****P < 0.0001.