Effects of DHA treatment on IκBα and nuclear NF-κB p65. By Western blot analysis, a basal level of IκB-α was detected in the spinal cord from sham-operated animals (A), whereas IκB-α levels were substantially reduced in SCI mice (A). DHA treatment reduced the SCI-induced IκB-α degradation (A). In addition, SCI caused a significant increase in nuclear NF-κB p65 compared with sham-operated mice (B). DHA treatment significantly reduced the phosphorylation of p65 on Ser536 and the translocation into the nucleus of NF-κB p65 (B). A representative blot of lysates obtained from each group is shown, and densitometry analysis of all animals is reported. Respective densitometry analysis of protein bands from three separated experiments is reported (***P < 0.001 vs. Sham; ###
P < 0.005 vs. SCI; ## P<0.05 vs.SCI).