Colocalization of GFAP/TNFα and Iba1/TNFα after SCI. Results are shown for (A-D, M-P) sham-operated mice, (E-H, R-U) mice with SCI and (I-L, V-Y) mice with SCI treated with DHA. Spinal cord sections were double stained with antibodies against GFAP (A,E,I, green), Iba1 (M,R,V, green) and TNFα (B,F,J,N,S,W, red). Spinal cord sections revealed increased astrogliosis (GFAP + cells) in SCI mice (E, R). GFAP immunoreactivity was reduced in DHA-treated mice (I, V). Yellow spots indicate co-localizations (H, U) and revealed a high colocalization between GFAP/TNFα and Iba1/TNFα double staining.