DHA treatment reduced Fas-ligand expression in the perilesional spinal cord tissue. No positive staining for Fas ligand was found in the spinal cord tissue collected from sham-operated mice (A, see densitometry analysis D). A substantial increase in Fas-ligand expression was found in inflammatory cells, and in white and gray matter of spinal cord tissues collected at 24 h after SCI (B, see densitometry analysis D). Spinal cord levels of Fas ligand were significantly attenuated by DHA treatment (C, see densitometry analysis D). (D) Densitometry analysis of immunocytochemistry photographs (n = 5 photos from each sample collected from all mice in each experimental group) for Fas ligand. Moreover Western blot analysis revealed an increased expression of Fas-L in the SCI group (E), whereas DHA treatment significantly reduced the increased levels of Fas-L (E). Figure is representative of at least three experiments performed on different experimental days. Data are mean ± SEM of ten mice for each group. *P < 0.01 vs Sham; °P < 0.01 vs SCI. # P<0.01 vs SCI; ND: not detectable.