Colocalization of GFAP/Bax and Iba1/Bax after SCI. Results are shown for (A-D, M-P) sham-operated mice, (E-H, R-U) mice with SCI and (I-L, V-Y) mice with SCI treated with DHA. Spinal cord sections were double stained with antibodies against GFAP (A,E,I, green), Iba1 (M,R,V, green) and Bax (B,F,J,N,S,W, red). Spinal cord sections revealed increased Bax expression in SCI mice (F,S). Bax expression was reduced in DHA-treated mice (J,W). Yellow spots indicate co-localizations (H,U) and revealed a high colocalization between GFAP/Bax and Iba1/Bax double staining.