Skip to main content
Figure 5 | Journal of Neuroinflammation

Figure 5

From: Activation of the omega-3 fatty acid receptor GPR120 mediates anti-inflammatory actions in immortalized hypothalamic neurons

Figure 5

The anti-inflammatory effect of docosahexaenoic acid (DHA) is abolished by the presence of BSA and replicated by the GPR120 agonist, GW9508. A. The presence of BSA (100 μM in H20) eliminated the anti-inflammatory effect of DHA pretreatment (100 μM in DMSO, 1 hr) on the transcriptional inflammatory response to TNFα treatment (10 ng/mL, 2 hr) as shown from the mRNA levels of IκBα (i) (DHA + H20: 0.20 ± 0.003 and DHA + BSA: 0.94 ± 0.09 IκBα/histone mRNA) and TNFα (ii) (DHA + H20; 0.51 ± 0.02 and DHA + BSA: 1.51 ± 0.09 TNFα/histone). B. The rHypoE-7 cells were pretreated with 100 μM GW9508 (GW) for 1 hr prior to the addition of 10 ng/mL TNFα and mRNA levels of IκBα (i) (DMSO; white bar: 1.61 ± 0.13 and GW; gray bar: 1.28 ± 0.05 IκBα/histone, n = 5 to 8) and TNFα (ii) (DMSO; white bar: 2.33 ± 0.21 and GW; gray bar: 1.31 ± 0.07 TNFα/histone, n = 5 to 8) were quantified by quantitative reverse transcriptase-PCR (qRT-PCR). GW significantly reduced the mRNA levels of both pro-inflammatory indicators relative to the DMSO vehicle. C. GPR120 co-immunoprecipitation (IP) with TAB1 shown by Western blots (WB) (i). DHA pretreatment significantly increased GPR120-TAB1 association (ii) (DMSO; white bar: 1.44 ± 0.40 and DHA; gray bar: 2.55 ± 0.49 GPR120/TAB1; n = 3 to 4). Data are shown as mean ± SEM; *P <0.05; ***P <0.01).

Back to article page