Figure 2
Time course of TLR 4 activation in the spine after I/R injury. (A) Representative immunofluorescence of TLR4. Prominent TLR4 immunoreactivation was observed in both dorsal horns of the spinal cord in operated rats. Spinal cord I/R-induced TLR4-up regulation increased with time and peaked at 12 and 48 h after surgery. Scale bars = 100 μm for the 100× and 50 μm for the 400× images. (B) Representative Western blot analysis and integrated density values (IDVs) of TLR4 activation. The IDVs of TLR4 in the I/R group with different reperfusion time points were calculated after normalizing against the Sham group and presented as relative protein expression units. The maximal of IDVs were observed at 12 and 48 h after surgery. All data are represented as means ± SEM (n = 8 per group). *P < 0.05 vs. Sham group.