Figure 5

Effects of TLR4 signaling on BSCB dysfunction after I/R injury. (A) Representative EB dye after intrathecal injection with TAK-242, MIP, and Resveratrol. Almost no red fluorescence was seen in spinal cord parenchyma in the Sham group at 12 and 48 h after injury. Much more red fluorescence could be seen in the I/R and Resveratrol groups at 12 h. These increased in intensity at 48 h in all groups after injury, especially in the gray matter of the I/R and MIP groups. Minimal EB red fluorescence was seen in the Sham and TAK groups at the above two time points. (B) Quantification data of EB content of spinal cord (μg/g). (C) Quantification of the water content of the spinal cord. All data are represented as mean ± SEM (n = 8 per group). Scale bars = 50 μm for 100× images. **P < 0.01 vs. Sham group; ## P < 0.01 vs. I/R group; &&P < 0.05 vs. TAK group.