Figure 6
Effects of TLR 4 signaling on NF-κB activation and inflammatory cytokines after I/R. (A) Representative Western blot and quantitative protein analysis of NF-κB in the spinal cord at 12 and 48 h after intrathecal pretreatment with TAK-242, MIP, and Resveratrol for 3 days. (B) The relative integral density values were calculated after normalizing against β-actin in each sample. (C) Quantification of IL-1β production in spinal cord at 12 and 48 h after I/R injury, as assessed by ELISA. **P < 0.01 vs. Sham group; ## P < 0.01 vs. I/R group; &&P < 0.05 vs. MIP group.