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Figure 2 | Journal of Neuroinflammation

Figure 2

From: Enhanced uptake of multiple sclerosis-derived myelin by THP-1 macrophages and primary human microglia

Figure 2

Increased uptake of MS myelin by THP-1 macrophages after 6 and 24 hours. (A) Flow-cytometric analysis of live cells defined by forward and sideward scatter (FSC and SSC) characteristics and the viability dye eFluor for uptake of pHrodo-labeled myelin after 6 and 24 hours of incubation. (B). Histograms showing uptake of 11 MS and 12 control myelin samples (pHrodo signal) after 6 and 24 hours. (C) THP-1 macrophages phagocytosed MS myelin more efficiently than control myelin after 6 and 24 hours. Both the percentage of cells that took up myelin and the amount of myelin taken up (geoMFI) was significantly higher when cells were incubated with MS myelin. Shown is the mean for each myelin sample in four independent experiments. Data were analyzed with the Mann–Whitney U test. HC, healthy control; MS, multiple sclerosis. * < 0.05; ** < 0.005; *** < 0.0005.

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