The number of splenocytes and splenic T-cells are not changed during chronic spinal cord injury (SCI). (A) Bar graph represents the mean ± SEM spleen weights of uninjured mice (CT) and T9-SCI mice at chronic phase after injury (SCI). n = 12 mice/group. Data are pooled across three independent experiments. (B) Bar graph represents the mean ± SEM of total splenocyte numbers for CT and SCI mice. n = 17 for CT, n = 19 for SCI. Data were pooled across four independent experiments. (C) Representative dot plots show the percentage of T-cells (CD45+CD3+) in live splenocytes (upper panels), as well as the percentages of CD4+ T-cells (CD4+CD8−, bottom right quadrant) and CD8+ T-cells (CD4−CD8+, upper left quadrant) in gated T-cells (bottom panels). (D) Bar graph represents the mean ± SEM number of splenic T-cells in CT and SCI mice. (E) Bar graph show the mean ± SEM numbers of splenic CD4+ T-cells and CD8+ T-cells in CT and SCI mice. Ten thousand events gated on live singlets were collected. n = 12 mice/group. Data are pooled across three independent experiments. No statistical difference was detected between the two groups. P > 0.05, two-tailed Student’s t-test.