Chronic spinal cord injury (SCI) increases the sympathetic activity in the spleen. (A) Western blot quantification of tyrosine hydroxylase (TH) in the spleens of uninjured mice (CT) and chronic SCI mice (SCI). Data are normalized to the expression of β-actin. Bar graph represents the mean ± SEM of TH expression in the spleen protein extract and are expressed as percentage of CT. n = 4 mice/group. Data represents two independent experiments. *P < 0.05, two-tailed Student’s t-test. (B) Bar graph represents the mean ± SEM of NE concentration (ng/mg) in the spleen homogenates from CT and SCI mice. n = 5 mice/group. *P < 0.05, one-tailed Student’s t-test.