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Figure 9 | Journal of Neuroinflammation

Figure 9

From: Chronic thoracic spinal cord injury impairs CD8+ T-cell function by up-regulating programmed cell death-1 expression

Figure 9

Norepinephrine (NE) up-regulates PD-1 expression on T-cells in vitro . Enriched splenic T-cells (106 cells/ml) were stimulated with 10 μM (NE) or its vehicle (Vehicle) in vitro for one to three days. Cells were collected and processed for flow cytometry. (A) Representative histograms show the 7-AAD fluorescence and the percentage of live cells (7-AAD) in gated singlet lymphocytes. (B) Bar graph shows the mean ± SEM percentage of live cells (7-AAD) following one to three days of in vitro culture with NE or vehicle. Experiments were performed in triplicate. *P < 0.05, ***P < 0.001, two-tailed Student’s t-test. (C) Representative dot plots show the percentage of PD-1+ cells in gated live CD4+ T-cells. (D) Bar graph represents the mean ± SEM number of CD4+ PD-1+ live cells per well after one and two days of NE or vehicle treatment. (E) Representative dot plots show the percentage of PD-1+ cells in gated live CD8+ T-cells. (F) Bar graph represents the mean ± SEM number of live CD8+PD-1+ cells per well after one and two days of NE or vehicle treatment. Ten thousand events gated on singlet lymphocytes were collected. Experiments were performed in triplicate. *P < 0.05, **P < 0.01, one-tailed Student’s t-test.

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