In vitro characterization of NSCs and OECs. Representative images of NSCs and OECs in vitro. (A) NSCs are tighter and larger 5 days after isolation from rat hippocampus, (B) nestin + neurosphere formation, and (C) neurons labeled with Hoechst 33342. (D) OECs present bipolar and multipolar cells with a three-dimensional, interlaced network after purified culture, (E) NGFR-p75+, and (F) labeled with CM-Dil. Scale bars are 30 μm in (A) and 50 μm in (D). CM-Dil, chloromethyl-benzamidodialkyl carbocyanine; NSC, neural stem cell; OEC, olfactory en sheathing cell.