Figure 4

5′ppp dsRNA activates RIG-1 signaling and increases expression of APP and Aβ. Representative immunoblot analysis of human cortical astrocyte lysates (A) of cells stimulated with 2 or 4 μg/ml of 5′ppp dsRNA for 18 hours. Non-stimulated cells were used as a control (Contr). Cell lysates were immunoblotted with antibodies against (B) RIG-1 and (C) P-IRF3. β-Actin was used as internal standard and control for protein loading. Data presented as mean ± SEM. *P < 0.05. N = 6. Representative immunoblot analysis of human cortical astrocyte lysates (D) of cells stimulated with 4 μg/ml of 5′ppp dsRNA for 18 hours. Non-stimulated cells were used as a control (Contr). Cell lysates were immunoblotted with antibodies against (E) APP and (F) Aβ. β-Actin was used as internal standard and control for protein loading. Data presented as mean ± SEM. *P < 0.05. N = 6.