Figure 5From: Age-related differences in neuroinflammatory responses associated with a distinct profile of regulatory markers on neonatal microglia Temporal expression of glial and immune cell markers after TLR4 stimulation. Brains from neonatal and weanling mice (described in Figure 3) were analyzed for (A-G) inflammatory cell markers as well as astrocytic and myeloid cell markers. An increase in T cell markers Cd3ϵ (A) and Cd8α (B), as well as neutrophil marker Ela2 (C) was not observed until 12 to 48 hours post infection. Gfap (D) mRNA was upregulated at 6 to 12 hours post infection with higher levels in weanling mice. In contrast, myeloid markers Itgax (E) and Cd80 (F) were higher in neonates than weanlings. Slamf7 (G), which is expressed by a number of immune cells, was also elevated in neonates compared with weanlings. Black symbols indicate data from LPS-stimulated mice, while gray symbols indicate PBS-treated controls. n = 4 to 6 mice per group per time point from combined experiments. Data are presented as mean ± SEM. Statistical analysis was completed by two-way analysis of variance with Tukey’s multiple comparisons test. Significant age-specific differences in the LPS response are as indicated: *P < 0.05, **P < 0.01, ***P < 0.001.Back to article page