SLAMF7 is strongly expressed by neonatal microglia. Cells were isolated as described in Figure 7. (A) SLAMF7 expression on microglia cells (dark blue gate in Figure 7) from an LPS-inoculated neonatal mouse (black line) or weanling mouse (gray line). Light gray line is from a sample that did not contain the anti-SLAMF7 antibody (no antibody negative control). Plots shown are from a single mouse but are consistent with data from 2 to 4 mice per age. (B) Average MFI of SLAMF7 expression on microglia cells from PBS or LPS-treated mice showing a higher level of SLAMF7 on neonatal microglia. Data are the mean ± SEM for 3 LPS-treated mice per group at 6 hpi. Only two data points are plotted for PBS-treated controls and thus represent a range of data rather than SEM. Data are representative of two replicate experiments, although different antibody concentrations were used in the second experiment (not shown). ***P < 0.001 for direct comparison of LPS-treated groups (C) SLAMF7 expression was not detected on the monocyte populations (combined cells from green and fuchsia gates in Figure 7) when compared with the negative (no antibody) control (light gray line).