Figure 1From: Epidermal growth factor receptor inhibitor ameliorates excessive astrogliosis and improves the regeneration microenvironment and functional recovery in adult rats following spinal cord injury PD168393 inhibited EGFR phosphorylation of astrocytes in an in vitro scratch wound model. Cultured astrocytes were immunostained for pEGFR, GFAP and DAPI in control (A1-A4), injury, (B1-B4) and PD168393 (40 μM) treatment groups (C1-C4) at 24 hours after injury (n = 5 in each group). (A1-C1) immunolabeling for pEFGR, (A2-C2), GFAP, (A3-C3) DAPI and (A4-C4) for co-localization of pEGFR, GFAP and DAPI. Scale bars = 100 μm. Representative Western blots of pEGFR and GFAP expression are shown (n = 3/group) (D). Semiquantitative analysis of pEGFR as a ratio of EGFR loading and semiquantitative measurements of GFAP were obtained by normalization to β-actin (E). *P < 0.05.Back to article page