PD168393 inhibited epidermal growth factor receptor (EGFR) phosphorylation of astrocytes after spinal cord injury (SCI) in rats. Spinal cord sections were immunostained for phosphorylated EGFR (pEGFR) and glial fibrillary acid protein (GFAP) at two weeks after SCI (n = 5/group). (A1-C1) GFAP, (A2-C2) pEFGR, and (A3-C3) co-localization of pEGFR and GFAP. Insets in panels (B1-C3) are shown at high magnification in panels (D1-E3), respectively. Scale bars = 500 μm in (C3) (applies to A1-C3); scale bars = 50 μm in (E3) (applies to D1-E3). Representative Western blots of pEGFR and EGFR expression (n = 3/group), and β-actin was a loading control (F). Semi-quantitative measurements were obtained by normalizing to β-actin (G). *P < 0.05.