PD168393 attenuated chondroitin sulfate proteoglycan (CSPG) production from reactive astrocytes after spinal cord injury (SCI). Double-immunofluorescent labeling for CSPGs and glial fibrillary acid protein (GFAP) at four weeks after SCI (n = 5/group). (A1-C1) GFAP, (A2-C2) CSPG, and (A3-C3) co-localization of CSPG and GFAP. Insets in panels (B1-C3) are shown at high magnification in panels (D1-E3). Scale bars = 500 μm in (C3) (applies to A1-C3); scale bars = 50 μm in (E3) (applies to D1-E3). Representative Western blots of GFAP and CS-56 expression (n = 3/group), and β-actin was a loading control (F). Semi-quantitative measurements were obtained by normalizing β-actin loading (G). *P < 0.05.