Figure 5From: Epidermal growth factor receptor inhibitor ameliorates excessive astrogliosis and improves the regeneration microenvironment and functional recovery in adult rats following spinal cord injury PD168393 attenuated chondroitin sulfate proteoglycan (CSPG) production from reactive astrocytes after spinal cord injury (SCI). Double-immunofluorescent labeling for CSPGs and glial fibrillary acid protein (GFAP) at four weeks after SCI (n = 5/group). (A1-C1) GFAP, (A2-C2) CSPG, and (A3-C3) co-localization of CSPG and GFAP. Insets in panels (B1-C3) are shown at high magnification in panels (D1-E3). Scale bars = 500 μm in (C3) (applies to A1-C3); scale bars = 50 μm in (E3) (applies to D1-E3). Representative Western blots of GFAP and CS-56 expression (n = 3/group), and β-actin was a loading control (F). Semi-quantitative measurements were obtained by normalizing β-actin loading (G). *P < 0.05.Back to article page