RvD1 enhances IL-4-induced PPARγ activation. (A) BV-2 cells were treated with IL-4 (10 ng/ml) for the indicated times, subcellular localization of PPARγ was evaluated using immunofluorescence staining. DNA was stained using 6-diamidino-2-phenylindole (DAPI) to visualize nuclei. The nuclear translocation of PPARγ was obvious 120 minutes after stimulation. Scale bars indicate 20 μm. (B, C) BV-2 cells were treated with RvD1 (100 nM), IL-4 (10 ng/ml), Boc-2 (10 μM) or a combination of them. Nuclear extracts were prepared 120 minutes after IL-4 treatment, Western blot or electrophoretic mobility shift assay (EMSA) were performed to detect PPARγ protein level in nucleus (B) or DNA binding activity (C). A representative result from three independent experiments is shown. Quantification of PPARγ was normalized by lamin B1. Data are presented as mean ± SEM for three independent experiments. Asterisks indicate statistically significant difference (*P < 0.05, **P < 0.01).