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Figure 5 | Journal of Neuroinflammation

Figure 5

From: Resolvin D1 promotes the interleukin-4-induced alternative activation in BV-2 microglial cells

Figure 5

Inhibition of STAT6 and PPARĪ³ impacts microglia alternative activation induced by IL-4 and RvD1. BV-2 cells were treated with leflunomide (10 Ī¼M) or GW9662 (1 Ī¼M) 30 minutes prior to administration of RvD1 (100 nM) and then stimulated with IL-4 (10 ng/ml) for 24 hours. Immunofluorescence assay for Arg1 (A) and Ym1 (B) indicated that the induction of Arg1 and Ym1 by RvD1 and IL-4 was disappeared when blocking STAT6 or PPARĪ³ signaling pathway. Scale bars indicate 20 Ī¼m. (C) The protein levels of Arg1 and Ym1 were measured by Western blot. A representative result from three independent experiments is shown. Quantification for Arg1 and Ym1 was normalized by Ī²-actin. Data are presented as meanā€‰Ā±ā€‰SEM for three independent experiments. Asterisks indicate statistically significant difference (*Pā€‰<ā€‰0.05, **Pā€‰<ā€‰0.01).

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