Systemic LPS injection results in an inflammatory response in the periphery and brain. (A) Plasma concentrations of (i) IL-1β, (ii) IL-6 and (iii) TNFα, 6, 12 and 48 hours following injection with LPS (100 μg i.p.) or vehicle. Data represent the mean ± the standard error of the mean (SEM). Significance was determined using two-way ANOVA: **P ≤ 0.01, ***P ≤ 0.001; n = 4/group. (B) Top enriched gene ontology classifications determined using DAVID Bioinformatics Resources. (C) Top significantly altered pathways determined using Ingenuity Pathway Analysis software. (B,C) Significance was calculated using Fisher’s exact test. (D) Comparison of entities identified using Partek Genomics Suite and Genespring GX software packages as being differentially expressed by ≥ 2-fold.