ISG induction in the brain and blood of LPS-treated mice. (A) Relative expression of ISGs in the brain and PBLs, 48 hours after LPS injection (100 μg i.p.) compared with vehicle injection. (B) Relative expression of ISGs in the brain, 6, 12 and 48 hours after LPS injection compared with vehicle injection. Gene expression normalized to TBP. Fold change calculated by comparing the normalized copy number in each sample with the mean of the vehicle-injected controls. Data represent mean ± SEM. Statistical significance was determined using two-way ANOVA: *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001; n = 4/group.