ISG induction in the brain and PBL of mice treated daily with LPS. Mice were injected daily with LPS (50 μg i.p.) or vehicle. (A,B) Relative gene expression in the brains and PBLs of mice, 2, 5 and 7 days following initial injection. (A) Relative expression of cytokines; (i) Il1b, (ii) Il6 and (iii) Tnfa. (B) Relative expression of target ISGs; (i) Cxcl10, (ii) Ifit1, (iii) Irf7, (iv) Oasl2 and (v) Gbp4. Gene expression normalized to TBP. Fold change was calculated by comparing the normalized copy number in each sample with the mean of the vehicle-injected controls. Data represent mean ± SEM. Statistical significance was determined using two-way ANOVA: *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001; n = 4/group.