Cellular localization of CXCL12 expression in spinal cord dorsal horn of sham and TCI rats. Double staining shows that CXCL12 (red) is colocalized with GFAP (green, middle), a marker for astrocytes, but not with NeuN (green, left), a marker for neurons or IBA1 (green, right), a marker for microglia. Tissues were collected 10 days after TCI. Two single-stained images were merged. Original magnification: 400× for all the confocal images showing the dorsal horn ipsilateral to TCI (bar = 50 μm).