Figure 5From: CXCL12 in astrocytes contributes to bone cancer pain through CXCR4-mediated neuronal sensitization and glial activation in rat spinal cord Effects of CXCL12 neutralizing antibody in spinal cord on mechanical and thermal hypersensitivity induced by TCI. (A, B) Intrathecal injection of CXCL12 neutralizing antibody (10 μg, but not 1 μg) partially and transiently reverses TCI-induced mechanical allodynia (A) and thermal hyperalgesia (B) in the feet ipsilateral to TCI. Pain behaviors were measured on postoperative day 10. #P < 0.05, ##P < 0.01 versus TCI + control IgG. (C, D) Intraspinal injection of CXCL12 neutralizing antibody (10 μg/10 μl, once a day for three consecutive days on days 3, 4, and 5 after TCI) delays TCI-induced induction of mechanical allodynia (C) and heat hyperalgesia (D). **P < 0.01 versus sham + control IgG. #P < 0.05, ##P < 0.01 versus TCI + control IgG. TCI was performed on day 0. The dose of control IgG was 10 μg/10 μl. Each administration is indicated by an arrow on the corresponding time point. Ten rats were included in each group.Back to article page