Figure 5

Effects of CXCL12 neutralizing antibody in spinal cord on mechanical and thermal hypersensitivity induced by TCI. (A, B) Intrathecal injection of CXCL12 neutralizing antibody (10 μg, but not 1 μg) partially and transiently reverses TCI-induced mechanical allodynia (A) and thermal hyperalgesia (B) in the feet ipsilateral to TCI. Pain behaviors were measured on postoperative day 10. #P < 0.05, ##P < 0.01 versus TCI + control IgG. (C, D) Intraspinal injection of CXCL12 neutralizing antibody (10 μg/10 μl, once a day for three consecutive days on days 3, 4, and 5 after TCI) delays TCI-induced induction of mechanical allodynia (C) and heat hyperalgesia (D). **P < 0.01 versus sham + control IgG. #P < 0.05, ##P < 0.01 versus TCI + control IgG. TCI was performed on day 0. The dose of control IgG was 10 μg/10 μl. Each administration is indicated by an arrow on the corresponding time point. Ten rats were included in each group.