Expression of FGF-2 and FGFRs in primary neurons and glial cells. (A) Expression of FGFRs as assessed by immunocytochemistry: FGFRs (green), MAP-2 (mature cortical neurons; red), CD11b (microglia; red), and GFAP (astrocytes; red). Scale bars, 10 μm in neurons and microglia, and 50 μm in astrocytes. (B) Expression of FGF-2 and FGFR1 to FGFR5 mRNA in mature cortical neurons (Neu), microglia (Mi) and astrocytes (Ast), as assessed by RT-PCR. GAPDH expression is used as a control.