The detection of FGF-2 in primary neurons and glial cells. (A) Neurons were treated with glutamate (Glu) or oligomeric amyloid β1-42 (oAβ) at the indicated concentrations. FGF-2 concentrations in the neuronal culture supernatants were measured using ELISAs at each time point. Results show the means with SEM (n = 3). Significant differences compared with untreated samples. *: P < 0.05, **: P < 0.01 (one-way ANOVA with Dunnett’s post-hoc test). (B) Astrocytes, microglia and neurons were treated with Glu (20 μM) or oAβ (5 μM) for 6 h. Other treatments of astrocytes (6 h) were LPS (1 μg/ml), TNF-α (20 ng/ml), IFN-γ (10 ng/ml), and a combination of all three. ELISA was then performed to detect FGF-2 concentration in the culture supernatants. The results show the means with SEM (n = 3). Significant differences compared with untreated samples in each cell type. *: P < 0.05, ***: P < 0.001, n.s.: not significant (one-way ANOVA with Dunnett’s post-hoc test).