Figure 2

NK cells promote neural necrosis via IFN-γ . (A): NK cells express IFN-γ in mouse ischemic cerebral tissue (bar = 20 μm); (B,C): Infiltration of IFN-γ/NK positive cells in mouse ischemic brain detected by FACS at different time points; (D,E): Percentage of neuronal cells undergoing apoptosis (A) and necrosis (N) after NK cells were co-cultured with or without IFN-γ; (F): Levels of IFN-γ mRNA detected in mouse ischemic hemispheres by PCR after NK cells depletion in vivo; (**P < 0.01, ***P < 0.001, compared with the corresponding values of the contralateral hemispheres).