Chemotactic effect of IP-10 on infiltrating NK cells during cerebral ischemia. (A,B): Expression of IP-10 in ipsilesional (A) and contralesional (B) hemispheres after stroke (magnification, 400×). (C): Significant differences between ipsilesional and contralesional hemispheres were observed. (D-G): Immunofluorescence detection of IP-10-positive cells (FITC) in mouse ischemic penumbra collected at different time points (bar = 20 μm). (H): Levels of IP-10 mRNA detected in mouse ischemic hemispheres by PCR; (I): Interaction between IP-10 and infiltrating NK cells; (J): Infiltration of NK cells detected in mouse ischemic tissue by FACS after IP-10 depletion in vivo. (**P < 0.01, ***P < 0.001, compared with the corresponding values of the contralateral hemispheres).