Figure 1

Time course of toll-like receptor-4 expression after lipopolysaccharide and restraint stress exposure. Protein levels of toll-like receptor (TLR)-4 in brain frontal cortex samples of (A) control and lipopolysaccharide-treated rats (LPS; 500 μg/kg), and (B) stressed rats during 2 hours (S2h), 6 hours (S6h) and 4 days (2 hours per day 4 days (6 hours per day)) (S4d). Data are representative of two experiments (n = 3 to 4 per group in each experiment). Two-tailed t-test (A) and one-way analysis of variance followed by Newman–Keuls post hoc test (B). **P < 0.01 versus control. O.D., optical density.