Flow cytometric analysis of leukocyte infiltration in the brains and spleens of WNV-infected WT and db / db mice. WT and db/db mice were infected with 10 PFU of WNV, and brains and spleens were harvested after extensive perfusion at day 8 after infection. Brain and spleen leukocytes were isolated and analyzed by flow cytometry. (A and B) Representative flow cytometry profiles demonstrating percentage of CD3+CD4+ T cells, CD3+CD8+ T cells, CD45+CD3- leukocytes and CD45+CD11b+ cells in the brain and spleen at day 8 after infection in WT and db/db mice. Data represents two independent experiments (n = 6 per group). (C and D) Total number of CD3+CD4+ T cells, CD3+CD8+ T cells, CD45+CD3- leukocytes and CD45+CD11b+ cells per brain and spleen were calculated. Data represents the mean ± SEM, representing two independent experiments (n = 6 per group). *P <0.05. PFU, plaque-forming units; SEM, standard error of mean.