Chemokines levels in the brains of WT and db / db mice after WNV infection. Brains were harvested from WT and db/db mice at indicated time points and homogenized as described in the Materials and methods section. Levels of chemokines as noted in the figure (A) CXCL10, (B) CXCL1, (C) CCL2, (D) CCL5, (E) CCL3, (F) G-CSF were measured using multiplex immunoassay and are expressed as the mean concentration (pg/g of tissues) ± SEM, representing two independent experiments (n = 7 per group). *P <0.05. SEM, standard error of mean.