Immunohistochemical analysis of CAM in the brains of WT and db / db mice after WNV infection. Cryopreserved brain sections from WNV-infected WT and db/db mice at day 8 after infection were stained with antibodies against E-selectin, ICAM-1, VCAM-1 (Red), and vWF (Green). The photomicrographs demonstrate representative images obtained from two independent experiments (n = 4 per group). Bars, 20 μm. ICAM-1, intercellular cell adhesion molecule 1; VCAM-1, vascular cell adhesion molecule 1; vWF, von Willebrand factor.