Journal of Neuroinflammation

Table 1 Primer sequences used for qRT-PCR

From: Reduced immune cell infiltration and increased pro-inflammatory mediators in the brain of Type 2 diabetic mouse model infected with West Nile virus

Gene (Accession No.)	Primer Sequence (5′-3′)	Amplicon
Gene (Accession No.)	Primer Sequence (5′-3′)	(bp)*	Tm (°C)^#
CD45 [GenBank: L36091]
Forward	GCCCAAACAAATTACACAT	107	58
Reverse	TTAGGCGTTTCTGGAATC
CD4 [GenBank: NM_013488]
Forward	GGAAGACTCTCAGACTTAT	79	57
Reverse	GAAGGTCACTTTGAACAC
CD8 [GenBank: NM_009857]
Forward	GTAATGAGCAGACTGTAAC	79	56
Reverse	CTATATGATGGGCAGACA
CD11b [GenBank: EF101557]
Forward	GCATGTCAAGAACAAGTA	133	56
Reverse	CTAAAGCCAGGTCATAAG
GFAP [GenBank: NM_010277]
Forward	GTGGATTTGGAGAGAAAG	177	56
Reverse	GTATTGAGTGCGAATCTC
ICAM-1 [GenBank: NM_010493]
Forward	ATAACTGGACTATAATCATTCTG	119	57
Reverse	AGCCTTCTGTAACTTGTAT
E-selectin [GenBank: M87862]
Forward	CATGACGTATGATGAAGC	98	57
Reverse	GATTGGAGTTAAGGTAGTTG
VCAM-1 [GenBank: NM_011693]
Forward	CTCTAGCAAGACCCTTTA	149	57
Reverse	CATCTTCACAGGCATTTC

*bp, base pair; ^# Tm, Melting temperature.

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ISSN: 1742-2094

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