Figure 4From: Macrophagic and microglial responses after focal traumatic brain injury in the female rat Immunohistochemistry of macrophagic and microglial markers in brain tissue after traumatic brain injury over time. Maximum immunofluorescence detection of markers of macrophagic and microglial polarization occurred by 5 days postinjury (DPI). (A) Representative anatomical localization of the regions of interest (ROIs; rectangles) quantified for the immunofluorescence of each marker. These ROIs were examined for the same anatomical slices from each animal. Scale bar = 3 mm. (B) Quantification of immunofluorescence in ROIs and representative immunofluorescent images at 1 DPI and 5 DPI. For a given time point, the column represents the average of all three ROIs for each slice, as shown in (A). Iba1, Ionized calcium-binding adapter molecule 1. Error bars indicate standard errors of the mean. *P < 0.05 for time point relative to 1 DPI. Scale bars = 100 μm.Back to article page