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Table 1 Antibody studies of M1 versus M2 phenotypes in neurotrauma a

From: Macrophagic and microglial responses after focal traumatic brain injury in the female rat

Study Injury model Species Sex Methods Cell type antibodies M1 markers M2 markers
Bedi et al. [40] CCI Mouse Male FC CD11b, CD45 CD16/CD32 CD206
Hsieh et al. [34] CCI Mouse Male FC, qRT-PCR on FC-sorted cells CD11b, CD45, F4/F80, Ly6G CD86, MHC II, YFP-IL-12p40 YFP-Arg1
Kigerl et al. [9] SCI Mouse Not specified IHC, qRT-PCR None CD16/CD32, CD86, iNOS, MHC II Arg1, CD206
Kumar et al. [15] CCI Mouse Male IHC, qRT-PCR CD11b, CD68, Iba1 MHC II Ym1
Lagraoui et al. [32] CCI Mouse Male qRT-PCR, multiplex None None None
Present study CCI Rat Female FC, IHC, qRT-PCR, multiplex CD68, Iba1 CD40, CD86 CD163
Wang et al. [35] CCI Mouse Male IHC, qRT-PCR Iba1 CD16/CD32 CD206
Zhang et al. [36] CCI Rat Not specified IHC CD68 None CD163
  1. a Arg1, Arginine 1; CCI, Controlled cortical impact; FC = Flow cytometry; Iba1, Ionized calcium-binding adapter molecule 1; IHC, Immunohistochemistry; IL, Interleukin; iNOS, Inducible nitric oxide synthase; MHC II, Major histocompatibility complex class II; qRT-PCR, Quantitative RT-PCR; SCI = Spinal cord injury, YFP = Yellow fluorescent protein.