Figure 2
From: Heat shock protein 60: an endogenous inducer of dopaminergic cell death in Parkinson disease
Hsp60 induction and release after MPP + treatment. (A) Hsp60 immunoblot analysis and quantification in cell lysates from SH-SY5Y after treatment with 100 μM MPP+ (at 6, 12, 24 and 48 hours) compared to control cells. (B) Hsp60 released by cultured SH-SY5Y cells treated by MPP+ (100 μM, at 6, 12, 24 and 48 hours) compared to control cultures. (C) MTT and lactate dehydrogenase (LDH) assay in SH-SY5Y cells exposed to MPP+ (100 μM, at 6, 12, 24 and 48 hours) compared to control cells. MTT assay (black bars), LDH assay (grey bars). *P < 0.05 versus corresponding controls.