Figure 3
From: Heat shock protein 60: an endogenous inducer of dopaminergic cell death in Parkinson disease
Induction of dopaminergic (DA) cell death by Hsp60. (A) Survival of TH+ neurons in mesencephalic neuron-glia cultures treated either with MPP+ (1 μM), hHsp60 (10 μg/ml) or a combination of both. (B) Survival of TH+ neurons in mesencephalic neuron-glia cultures exposed to native hHsp60 (10 μg/ml) alone or in the presence of thelipopolysaccharide antagonist PMBS (10 μg/ml), or to trypsinized hHsp60. hHsp60 was also applied to AraC (2 μM)-treated cultures, a treatment that eliminates most of glial cells from mesencephalic cultures. *P < 0.05 versus corresponding controls. #P < 0.05 versus hHsp60 treatment.