Figure 4

Characteristics of inflammatory cells and molecules in retina after systemic lipopolysaccharide. A, B. Flow cytometry revealed that the percentage of major histocompatibility complex (MHC) class II^loCD68^loCD11b^loCD11c^hi cells in the retina was increased in the LPS-treated group on P7, and normalized on P14. There was no difference in the percentage of CD11b^hi cells between the LPS- and normal saline (NS)-treated retinas. C. Real time RT-PCR showed that the levels of TNF-α, IL-1β, and IL-12a transcripts were increased in the retina of the LPS-treated group on P7, and returned to baseline on P14. Also, the level of thrombospondin (TSP)-1, an anti-angiogenic and pro-apoptotic factor, was significantly increased in the retina of the LPS-treated rats, while the level of VEGF transcript was not altered. *P <0.05; **P <0.01; ns, not significant.