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Figure 4 | Journal of Neuroinflammation

Figure 4

From: Roles of NFκB-miR-29s-MMP-2 circuitry in experimental choroidal neovascularization

Figure 4

TNFα reduced miR-29 s, which was reversed by NFκB decoy. ARPE-19 cells were cultured in six-well dishes and transfected with NFκB decoy ODNs (50 nM) or scramble ODNs (50 nM) when the cell densities reached approximately 70% confluency. The ARPE-19 cells were then treated with TNFα (10 ng/mL) for 12 hours and miR-29 s levels were determined by RT-qPCR. The values of control were set as 100% and the results from treatments were normalized to control. The results shown are mean (±SEM) from at least three independent experiments with each condition per experiment from quadruplicate cultures. *P < 0.05 versus control. NFκB, nuclear factor of kappa light polypeptide gene enhancer in B-cells; ODN, oligodeoxynucleotide; TNFα, tumor necrosis factor alpha.

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