Figure 3

Photomicrographs of immature and mature oligodendrocytes and caspase-3 labeled apoptotic cells in the PVWM. Photomicrographs showing CNPase immunolabeling (A-D), and cleaved caspase-3 (ASP175) immunolabeling (E-H) in the PVWM in Sal-Sham (row 1; A, E), LPS-Sham (row 2; B, F), Sal-Asp (row 3; C, G) and LPS-Asp (bottom; D, H). Arrows show examples of labeled cells. Note the reduction in CNPase positive cells and increase in activated caspase-3 labelling after LPS-Sham and Sal-Asp. Surviving CNPase positive cells show stunted processes. LPS-Asp treatment was associated with recovery of CNPase positive immature and mature oligodendrocytes to Sal-Sham values, and reduced numbers of activated caspase-3 positive cells compared with Sal-Asp but not LPS-Sham. Scale bar = 20 μm. Asp, asphyxia; CNPase, 2′,-3′-Cyclic-nuceotide 3′-phosphodiesterase; LPS, lipopolysaccharide; PVWM, periventricular white matter; Sal, saline.