Figure 4
Effect of the CAMKII inhibitor on p-p38 and p-JNK levels in incubated arteries (A) Fluorescence intensity and (B) expression of p-p38 and p-JNK in the vascular smooth muscle cell layer of cerebral arteries incubated for 24 hours in presence or absence of the CaMKII inhibitor KN93 added at 0 or 6 hours after initiating incubation. (A, D) Arteries incubated in the presence of vehicle for 24 hours were used as the control and treatments compared to this level of expression (set as 100%). (B, E) KN93 added at 0 hours, (C, F) KN93 added at 6 hours. Data are presented as mean ± SEM relative to control, n = 5, *P <0.05. ^^P <0.01 represents significant difference in p-p38 protein level between 0 and 6 hours. Negative controls (omission of primary antibody) for Cy2 -conjugated donkey anti-rabbit and Texas-Red-conjugated donkey anti-mouse. Calcium calmodulin-dependent protein kinase II (CAMKII), phosphorylated c-Jun N-terminal kinase (p-JNK).