Figure 6

Effect of the MEK1/2 inhibitor or the CAMKII inhibitor on TNFR1 levels in incubated arteries (A) Fluorescence intensity and (B) expression of TNFR1 in the vascular smooth muscle cell layer of cerebral arteries incubated for 24 hours in presence or absence of the MEK1/2 inhibitor U0126 or the CAMKII inhibitor KN93, added at 0 or 6 hours after initiating incubation. Data are presented as mean ± SEM relative to 24 hours incubation in presence of vehicle (control). n = 5. *P <0.05, **P <0.01. ^^P <0.01 represents significant difference in TNFR1 protein level between 0 and 6 hours in arteries treated with U0126. Calcium calmodulin-dependent protein kinase II (CAMKII), tumor necrosis factor receptor 1 (TNFR1).