Figure 5

A single co-injection of D-mannose with low dose pDNA-IL-10 produces enduring reversal of allodynia. (A and B) after baseline (BL) assessment, bilateral hindpaw responses from sham versus chronic constriction injury (CCI) treated animals revealed bilateral allodynia through day 10 (ipsilateral, F_(4,32) = 32.07, P < 0.0001; contralateral, F_{(5, 32)} = 38.78; P < 0.0001). After testing on day 10, sham rats received a single i.t. saline injection (open inverted triangles; n = 8) or D-mannose (50 μg; closed triangles; n = 4), and CCI rats received a single i.t. co-injection of: (1) saline alone (open diamonds; n = 6), (2) equivolume saline with pDNA-IL-10 (1 μg; open triangles; n = 3), (3) D-mannose (50 μg) with ‘control pDNA’, which lacks the IL-10 gene (control pDNA; open circles; n = 7), or (4) D-mannose with pDNA-IL-10 (closed squares; n = 10). Sham treated groups (saline or D-mannose) and CCI-saline, or CCI-D-mannose with control pDNA resulted in no change from allodynia while D-mannose co-injected with of pDNA-IL-10 (1 μg) resulted in full reversal of allodynia throughout the three-week time course (ipsilateral F_{(5, 238)} = 60.23; P <0.001; contralateral F_{(5, 224)} = 22.18, P < 0.001).