Figure 5

No effects of lidocaine on IL-1β-induced inhibition of TEA-activated GluR1 trafficking. The hippocampus was harvested from four-month-old control Fischer 344 rats. Freshly prepared 300 μm coronal hippocampal slices were incubated with or without 10 μM lidocaine, or 10 μM lidocaine plus 3 ng/ml IL-1β for one hour at 37°C and then with 25 mM TEA in the presence of 1 mg/ml sulfo-NHS-SS-biotin, a biotinylation reagent, for 10 minutes at 37°C. The homogenates of the hippocampal slices were used for western blotting of phospho-GluR1 and total GluR1 and the biotinylated fraction was used for western blotting of GluR1. A: representative Western blot images, B: biotinylated GluR1, C: phospho-GluR1 at ser845, and D: total GluR1. Results are means ± SD (n = 6). *P <0.05 compared with the corresponding control. GAPDH: glyceraldehydes 3-phosphate dehydrogenase, P-GluR1: phospho-GluR1, S-GluR1: cell surface GluR1, TEA, tetraethylammonium; T-GluR1: total GluR1.