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Figure 6 | Journal of Neuroinflammation

Figure 6

From: Deficiency of complement receptors CR2/CR1 in Cr2 -/-mice reduces the extent of secondary brain damage after closed head injury

Figure 6

Selected pro- (Fas, FasL, and Bax) and anti-apoptotic (Bcl-2) mediator levels in brain homogenates. Samples were analyzed by western blots of injured (left) and non-injured (right) hemispheres at seven days after traumatic brain injury (TBI) or sham operation. Equal concentrations of protein (50 μg per lane) were loaded on SDS-Page, and consistent loading was confirmed by β-Actin control blotting. Mouse-specific primary antibodies against Fas, FasL, Bax, and Bcl-2 were used on nitrocellulose membranes and visualized by a colorimetric assay using alkaline-phosphatase. No differences in mediator levels were detected between the groups at any time-point assessed.

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